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polyclonal anti-src [py418] phospho-specific antibody  (Thermo Fisher)


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    Thermo Fisher polyclonal anti-src [py418] phospho-specific antibody
    Polyclonal Anti Src [Py418] Phospho Specific Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polyclonal anti-src [py418] phospho-specific antibody/product/Thermo Fisher
    Average 90 stars, based on 1 article reviews
    polyclonal anti-src [py418] phospho-specific antibody - by Bioz Stars, 2026-03
    90/100 stars

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    ( a ) MDCK type I cells harbouring Src-EGFP were mixed with wild-type cells at a ratio of 35:1 and preincubated with the 100 μM S3i-201 (S3i), 20 μM stattic (Sta), 20 μM marimastat (Mari), or 400 nM ilomastat (Ilo) for 2 h and then incubated with Dox for 24 h. ( b ) Two type I clones harbouring Src-EGFP and STAT3-Y705F (DN) were mixed with wild-type cells at a ratio of 35:1 and incubated with Dox for 24 h. Cell behaviour was assessed by using the criteria . ( c, d ) Type I cells harbouring Src-EGFP were mixed with wild-type cells at a ratio of 10:1 and incubated with Dox for 24 h. Wild-type and Src-EGFP harbouring cells were separately incubated with Dox for 24 h, and cell lysates were mix at a ratio of 10:1. These cell lysates were subjected to immunoblotting analysis using the indicated antibodies. Relative intensity of p-Src <t>(pY418)/GFP,</t> p-STAT3 (pY705)/STAT3, and pY1000/Gapdh were calculated by setting the value for lysate mix to one. ( e, f ) Type I and II harbouring Src-EGFP were incubated in the presence of Dox for indicated time periods. Lysate from these cells were analysed by immunoblotting using indicated antibodies. Relative ratio of p-Src/GFP, p-STAT3/STAT3, and pY1000/Gapdh were calculated by setting the value for 24 h-treated type I cells to one. The mean ratios ± SDs were obtained from three independent experiments. *, P < 0.05; **, P < 0.01; ***, P < 0.001; two-way ANOVA was calculated compared with the Dox-treated cells (a) or mock-transfected cells (b), and two-tailed t -test was calculated (d, f).
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    ( a ) MDCK type I cells harbouring Src-EGFP were mixed with wild-type cells at a ratio of 35:1 and preincubated with the 100 μM S3i-201 (S3i), 20 μM stattic (Sta), 20 μM marimastat (Mari), or 400 nM ilomastat (Ilo) for 2 h and then incubated with Dox for 24 h. ( b ) Two type I clones harbouring Src-EGFP and STAT3-Y705F (DN) were mixed with wild-type cells at a ratio of 35:1 and incubated with Dox for 24 h. Cell behaviour was assessed by using the criteria . ( c, d ) Type I cells harbouring Src-EGFP were mixed with wild-type cells at a ratio of 10:1 and incubated with Dox for 24 h. Wild-type and Src-EGFP harbouring cells were separately incubated with Dox for 24 h, and cell lysates were mix at a ratio of 10:1. These cell lysates were subjected to immunoblotting analysis using the indicated antibodies. Relative intensity of p-Src <t>(pY418)/GFP,</t> p-STAT3 (pY705)/STAT3, and pY1000/Gapdh were calculated by setting the value for lysate mix to one. ( e, f ) Type I and II harbouring Src-EGFP were incubated in the presence of Dox for indicated time periods. Lysate from these cells were analysed by immunoblotting using indicated antibodies. Relative ratio of p-Src/GFP, p-STAT3/STAT3, and pY1000/Gapdh were calculated by setting the value for 24 h-treated type I cells to one. The mean ratios ± SDs were obtained from three independent experiments. *, P < 0.05; **, P < 0.01; ***, P < 0.001; two-way ANOVA was calculated compared with the Dox-treated cells (a) or mock-transfected cells (b), and two-tailed t -test was calculated (d, f).
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    Antibodies Used in This Study

    Journal: Inflammation

    Article Title: Focal Adhesion Kinase Activity and Localization is Critical for TNF-α-Induced Nuclear Factor-κB Activation

    doi: 10.1007/s10753-020-01408-5

    Figure Lengend Snippet: Antibodies Used in This Study

    Article Snippet: pY418 Src , Cell Signal Technology, 2101 , Rabbit , 1:2000 (WB) , AB_331697.

    Techniques: Recombinant

    ( a ) MDCK type I cells harbouring Src-EGFP were mixed with wild-type cells at a ratio of 35:1 and preincubated with the 100 μM S3i-201 (S3i), 20 μM stattic (Sta), 20 μM marimastat (Mari), or 400 nM ilomastat (Ilo) for 2 h and then incubated with Dox for 24 h. ( b ) Two type I clones harbouring Src-EGFP and STAT3-Y705F (DN) were mixed with wild-type cells at a ratio of 35:1 and incubated with Dox for 24 h. Cell behaviour was assessed by using the criteria . ( c, d ) Type I cells harbouring Src-EGFP were mixed with wild-type cells at a ratio of 10:1 and incubated with Dox for 24 h. Wild-type and Src-EGFP harbouring cells were separately incubated with Dox for 24 h, and cell lysates were mix at a ratio of 10:1. These cell lysates were subjected to immunoblotting analysis using the indicated antibodies. Relative intensity of p-Src (pY418)/GFP, p-STAT3 (pY705)/STAT3, and pY1000/Gapdh were calculated by setting the value for lysate mix to one. ( e, f ) Type I and II harbouring Src-EGFP were incubated in the presence of Dox for indicated time periods. Lysate from these cells were analysed by immunoblotting using indicated antibodies. Relative ratio of p-Src/GFP, p-STAT3/STAT3, and pY1000/Gapdh were calculated by setting the value for 24 h-treated type I cells to one. The mean ratios ± SDs were obtained from three independent experiments. *, P < 0.05; **, P < 0.01; ***, P < 0.001; two-way ANOVA was calculated compared with the Dox-treated cells (a) or mock-transfected cells (b), and two-tailed t -test was calculated (d, f).

    Journal: bioRxiv

    Article Title: Src activation in lipid rafts confers epithelial cells with invasive potential to escape from apical extrusion during cell competition

    doi: 10.1101/2021.05.29.446275

    Figure Lengend Snippet: ( a ) MDCK type I cells harbouring Src-EGFP were mixed with wild-type cells at a ratio of 35:1 and preincubated with the 100 μM S3i-201 (S3i), 20 μM stattic (Sta), 20 μM marimastat (Mari), or 400 nM ilomastat (Ilo) for 2 h and then incubated with Dox for 24 h. ( b ) Two type I clones harbouring Src-EGFP and STAT3-Y705F (DN) were mixed with wild-type cells at a ratio of 35:1 and incubated with Dox for 24 h. Cell behaviour was assessed by using the criteria . ( c, d ) Type I cells harbouring Src-EGFP were mixed with wild-type cells at a ratio of 10:1 and incubated with Dox for 24 h. Wild-type and Src-EGFP harbouring cells were separately incubated with Dox for 24 h, and cell lysates were mix at a ratio of 10:1. These cell lysates were subjected to immunoblotting analysis using the indicated antibodies. Relative intensity of p-Src (pY418)/GFP, p-STAT3 (pY705)/STAT3, and pY1000/Gapdh were calculated by setting the value for lysate mix to one. ( e, f ) Type I and II harbouring Src-EGFP were incubated in the presence of Dox for indicated time periods. Lysate from these cells were analysed by immunoblotting using indicated antibodies. Relative ratio of p-Src/GFP, p-STAT3/STAT3, and pY1000/Gapdh were calculated by setting the value for 24 h-treated type I cells to one. The mean ratios ± SDs were obtained from three independent experiments. *, P < 0.05; **, P < 0.01; ***, P < 0.001; two-way ANOVA was calculated compared with the Dox-treated cells (a) or mock-transfected cells (b), and two-tailed t -test was calculated (d, f).

    Article Snippet: Anti-Src pY418 (44-655G) and anti-GFP (A6455) were purchased from Thermo Fisher Scientific.

    Techniques: Incubation, Clone Assay, Western Blot, Transfection, Two Tailed Test